Difference between revisions of "Part:BBa K1755001"

(Sequence)
(Sequence)
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== Sequence ==
 
== Sequence ==
         10        20        30         40        50        60        70        80        90        100
+
         10        20        30         40        50        60        70        80        90        100
 
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM  
 
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM  
      110        120        130        140        150        160        170        180        190        200
+
        110        120        130        140        150        160        170        180        190        200
 
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR  
 
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR  
      210  
+
        210  
 
TIEDLVAYRQ AHERKAS
 
TIEDLVAYRQ AHERKAS
 
[[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]]
 
[[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]]

Revision as of 06:49, 27 August 2015

ribB coding sequence

ribB,the 3,4 dihydroxy-2-butanone-4-phosphate synthase。 We get this part from E.coli genome by PCR. It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.

Usage and Biology

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.

This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.

Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.


Sequence

       10         20         30          40         50         60         70         80         90        100

TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM

       110        120        130        140        150        160        170        180        190        200

GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR

       210 

TIEDLVAYRQ AHERKAS

Normal E.coli(left one)and E.coli with ribB(right one)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]