Difference between revisions of "Part:BBa K1755001"

Revision as of 06:32, 27 August 2015

ribB coding sequence

ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase。 We get this part from E.coli genome by PCR. It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.

Usage and Biology

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

Normal E.coli(left one)and E.coli with ribB(right one)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 387
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 277
Illegal AgeI site found at 319
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1755001 short</partinfo>
-[[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]]
-----
 ribB&#65292;the 3,4 dihydroxy-2-butanone-4-phosphate synthase&#12290;
@@ Line 12: / Line 9: @@
 After the addition of ribB, the bacteria will be yellow.
-===Usage and Biology===
+==='''Usage and Biology'''===
 The protein RibB catalyzes the conversion of
@@ Line 26: / Line 23: @@
 D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
+[[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]]
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