Difference between revisions of "Part:BBa K1585101:Design"

(→‎Design Notes)
 
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Assambly of [https://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [https://parts.igem.org/Part:BBa_R0010 R_0010]
 
Assambly of [https://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [https://parts.igem.org/Part:BBa_R0010 R_0010]
  
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The "lacI binding site" of Part [https://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [https://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same.  
+
The "lacI binding site" of Part [https://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [https://parts.igem.org/Part:BBa_J33207 BBa_J33207].
  
 
===References===
 
===References===

Latest revision as of 17:03, 26 August 2015

Anderson Promoter with lacI binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.

The part is shipped in backbone J61002 because the functionality is directly visible by adding IPTG. RFP (E1010) should get expressed then.

K15751xx design.png

Source

Assambly of J23100 and lacI-binding-site of R_0010

The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207.

References