Difference between revisions of "Part:BBa J31007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid. | + | This sequence was amplified from <partinfo>pSB1AT3</partinfo> and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid. |
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| + | This part was PCR amplified from <partinfo>pSB1AT3</partinfo> using the following primers. The primers have non-annealing 5'- extensions that introduce an <font color='red'>XbaI site</font> to the left and a <font color='blue'>SpeI site</font> to the right of the coding region. Primer annealing sites are shown in bold. | ||
| + | <br>Forward: 5’ GCAT<font color='red'>TCTAG <b>A</b></font><b>TGAAATCTAACAATGCGCTCATC</b> | ||
| + | <br>Reverse: 5’ ATGC<font color='blue'>ACTAG <b>T</b></font><b>TAGGTCGAGGTGGCCCGGC</b> | ||
===Source=== | ===Source=== | ||
Latest revision as of 21:52, 6 December 2006
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 144
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 290
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 316
Illegal NgoMIV site found at 684
Illegal NgoMIV site found at 844 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid.
This part was PCR amplified from pSB1AT3 using the following primers. The primers have non-annealing 5'- extensions that introduce an XbaI site to the left and a SpeI site to the right of the coding region. Primer annealing sites are shown in bold.
Forward: 5’ GCATTCTAG ATGAAATCTAACAATGCGCTCATC
Reverse: 5’ ATGCACTAG TTAGGTCGAGGTGGCCCGGC