Difference between revisions of "Part:BBa K1720002"

Revision as of 14:08, 26 August 2015

Hypoxia-induced promotor

This part is a hypoxia response element.When the cells suffer from hypoxia situation this element will begin to work.It will activate the downstream gene expression.The hypoxia response element is a minimal cis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian cells.

This element with a GFP reporter was then transfected into HEK293 cells .Then we treat the cells with sodium hyposulfite, an reagent that leads to hypoxia. The negative control was HEK293 cells that treat with PBS and we did not detect green fluorescence signal.But we detected green fluorescence signal in the experimental group ,which meant that this element work under hypoxia situation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 41
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 41
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 41
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 41
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1720002 short</partinfo>
-This part is a hypoxia response element.
-The hypoxia response element is a minimal cis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian cells.
+This part is a hypoxia response element.When the cells suffer from hypoxia situation this element will begin to work.It will activate the downstream gene expression.The hypoxia response element is a minimal cis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian cells.
-This element with a GFP reporter was then transfected into HEK293 cells.We use Vivo green fluorescence signal was observed under fluorescence microscope,
+This element with a GFP reporter was then transfected into HEK293 cells .Then we treat the cells with sodium hyposulfite, an reagent that leads to hypoxia. The negative control was HEK293 cells that treat with PBS and we did not detect green fluorescence signal.But we detected green fluorescence signal in the experimental group ,which meant that this element work under hypoxia situation.
 <!-- Add more about the biology of this part here