Difference between revisions of "Part:BBa K1110003:Design"
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===References=== | ===References=== | ||
Revision as of 15:16, 25 August 2015
Mambalgin-1 cDNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For the design, we used assembly standard 25 and removed a start codon from the sequence. Using the pGAPZα expression system, the GSU team has been working to express the protein in Pichia Pastoris. The pGAPZα vector includes an alpha secretion, MYC epitome, and 6xHis tag region. This sequence is optimized for this expression system.
Source
Dendroaspis polylepis Synthesized by IDT