Difference between revisions of "Part:BBa K1585101:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We choosed part BBa_R0010 because we assumed older parts would be more reliable. | + | We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. |
The part is shipped in backbone [https://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([https://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then. | The part is shipped in backbone [https://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([https://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then. | ||
Revision as of 12:55, 25 August 2015
Anderson Promoter with lacI binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone J61002 because the functionality is directly visible by adding IPTG. RFP (E1010) should get expressed then.
Source
Assambly of J23100 and lacI-binding-site of R_0010
The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207 although they should be same.