Difference between revisions of "Part:BBa K1585100:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
The "lacI binding site" of Part [https://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [https://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same.  
 
The "lacI binding site" of Part [https://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [https://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same.  
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We choosed part BBa_R0010 because we assumed older parts would be reliable.
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We choosed part BBa_R0010 because we assumed older parts would be more reliable.
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===Source===
 
===Source===

Revision as of 17:02, 24 August 2015


Anderson Promoter with lacI binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207 although they should be same. We choosed part BBa_R0010 because we assumed older parts would be more reliable.

Source

Assambly of J23100 and lacI-binding-site of R_0010

References