Difference between revisions of "Part:BBa K1585101:Design"
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===Design Notes=== | ===Design Notes=== | ||
The "lacI binding site" of Part [https://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [https://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. | The "lacI binding site" of Part [https://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [https://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. | ||
| − | We choosed part BBa_R0010 because we assumed older parts would be reliable. | + | We choosed part BBa_R0010 because we assumed older parts would be more reliable. |
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===Source=== | ===Source=== | ||
Revision as of 17:01, 24 August 2015
Anderson Promoter with lacI binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207 although they should be same. We choosed part BBa_R0010 because we assumed older parts would be more reliable.
Source
Assambly of J23100 and lacI-binding-site of R_0010