Difference between revisions of "Part:BBa K1607010:Design"

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===Source===
 
===Source===
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    This coding sequence is synthesized by LCR assmebly using 36 oilgos.
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The obtainment of DNA sequence:   
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  To get this coding sequence, we found the AA sequence of this SCFV on NCBI first (PDB: 3H3B_B). The AA sequence was then reverse translated into DNA sequence. The DNA sequence was analyzed by gene2oligo and 36 oligos were given as a result. We synthesized the 36 oligos and obtained the 601bp fragment of SCFV coding sequence by LCR assembly using these oligos.
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===References===
 
===References===

Revision as of 05:11, 24 August 2015


The coding sequence of the SCFV of anti-p185her2/neu antibody chA21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 370
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The termination codon: 

   Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.

Source

   This coding sequence is synthesized by LCR assmebly using 36 oilgos.

References