Difference between revisions of "Help:Plasmid backbones"

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[[image:PlasmidDNA.png]]
 
[[image:PlasmidDNA.png]]
 
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell]. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.  
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell] independently of the chromosomal DNA. They are often acquired by bacteria and can bestow the benefit of antibiotic resistance or production of novel proteins. Plasmids can incorporate BioBrick devices and systems into cells via transformation.  
  
 
*[http://en.wikipedia.org/wiki/Transformation_(genetics) '''Transformation''']
 
*[http://en.wikipedia.org/wiki/Transformation_(genetics) '''Transformation''']
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===Antibiotic Resistance===
 
===Antibiotic Resistance===
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By incorporating an antibiotic-resistance gene on a plasmid, engineered-cells are able to be selected for using the appropriate antibiotic. The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor").  For more information on which plasmids carry these resistances, click the [[Help:Plasmids/Nomenclature |nomenclature]] documentation section.     
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By incorporating an antibiotic-resistance gene on a plasmid, you can select for engineered cells using the appropriate antibiotic. The most commonly used antibiotics are ampicillin ("Amp"), kanamycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor").  For more information on which plasmids carry these resistance genes, click the [[Help:Plasmids/Nomenclature |nomenclature]] documentation section.     
  
 
<b>
 
<b>
 
All of our plasmids are available through the use of [[Part:BBa_P1010| BBa_P1010]].  (Information on how to use that part is available on its part page)</b>
 
All of our plasmids are available through the use of [[Part:BBa_P1010| BBa_P1010]].  (Information on how to use that part is available on its part page)</b>

Revision as of 21:51, 29 November 2006

PlasmidDNA.png

Plasmids are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. They are often acquired by bacteria and can bestow the benefit of antibiotic resistance or production of novel proteins. Plasmids can incorporate BioBrick devices and systems into cells via transformation.

  • [http://en.wikipedia.org/wiki/Transformation_(genetics) Transformation]
  • Types of Plasmids - What's a construction plasmid? Does "plasmid" mean vector backbone only?
  • Plasmid features - Features of plasmid parts & their feature tables of plasmids mean
  • Plasmid Naming - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).

Antibiotic Resistance

By incorporating an antibiotic-resistance gene on a plasmid, you can select for engineered cells using the appropriate antibiotic. The most commonly used antibiotics are ampicillin ("Amp"), kanamycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor"). For more information on which plasmids carry these resistance genes, click the nomenclature documentation section.

All of our plasmids are available through the use of BBa_P1010. (Information on how to use that part is available on its part page)