Difference between revisions of "Part:BBa K310004:Experience"
Sarahwideman (Talk | contribs) |
Sarahwideman (Talk | contribs) (→Applications of BBa_K310004) |
||
| Line 4: | Line 4: | ||
===Applications of BBa_K310004=== | ===Applications of BBa_K310004=== | ||
| + | This part doesn’t work. The part is not the described MicF target, rather it appears to be a Cu-responsive transcriptional regulator from Salmonella enterica. | ||
| + | |||
| + | Restriction analysis with EcoRI and PstI shows an insert that is much larger than the expected 190 bp. | ||
| + | |||
| + | [[File:K310004 restriction analysis Stockholm 2015.png|border|Restriction Analysis]] | ||
| + | |||
| + | '''Left''': restriction analysis gel of K310004 in pSB1C3. | ||
| + | '''Middle''': Simulated gel (SnapGene) with K310004 sequence provided by iGEM Team UIUC Illinois 2010. | ||
| + | '''Righ'''t: Simulated gel (SnapGene) with sequence provided by iGEM Team Stockholm 2015 | ||
| + | |||
| + | Well 2: Positive control (134 bp) | ||
| + | Well 3: K310004 in pSB1C3 digested with EcoRI | ||
| + | Well 4: K310004 in pSB1C3 digested with EcoR and PstI | ||
| + | Well 5: K310004 in pSB1C3 digested with EcoRI | ||
| + | Well 6: K310004 in pSB1C3 digested with EcoR and PstI | ||
| + | |||
| + | We therefore sequenced the part and found that it is a 684 bp BioBrick flanked by the BioBrick prefix and suffix. BLAST alignment of the part showed that it probably is a Cu-responsive transcriptional regulator from Salmonella enterica. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:01, 18 August 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K310004
This part doesn’t work. The part is not the described MicF target, rather it appears to be a Cu-responsive transcriptional regulator from Salmonella enterica.
Restriction analysis with EcoRI and PstI shows an insert that is much larger than the expected 190 bp.
Left: restriction analysis gel of K310004 in pSB1C3. Middle: Simulated gel (SnapGene) with K310004 sequence provided by iGEM Team UIUC Illinois 2010. Right: Simulated gel (SnapGene) with sequence provided by iGEM Team Stockholm 2015
Well 2: Positive control (134 bp) Well 3: K310004 in pSB1C3 digested with EcoRI Well 4: K310004 in pSB1C3 digested with EcoR and PstI Well 5: K310004 in pSB1C3 digested with EcoRI Well 6: K310004 in pSB1C3 digested with EcoR and PstI
We therefore sequenced the part and found that it is a 684 bp BioBrick flanked by the BioBrick prefix and suffix. BLAST alignment of the part showed that it probably is a Cu-responsive transcriptional regulator from Salmonella enterica.
User Reviews
UNIQ92a9097d6306351a-partinfo-00000000-QINU UNIQ92a9097d6306351a-partinfo-00000001-QINU