Difference between revisions of "Part:BBa K1725020"
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<partinfo>BBa_K1725020 short</partinfo> | <partinfo>BBa_K1725020 short</partinfo> | ||
The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000. | The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000. | ||
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| + | https://static.igem.org/mediawiki/2015/thumb/a/ad/2015-08-05_10.50.30_cropped.jpg/800px-2015-08-05_10.50.30_cropped.jpg | ||
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| + | From left to right: I13500(B0034.E0040); R0040.I13500; K1725000.I13500; K1725020.I13500 all in pSB3K3 in DH5a cells. | ||
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Revision as of 11:49, 7 August 2015
SrpR repressible promoter
The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.
From left to right: I13500(B0034.E0040); R0040.I13500; K1725000.I13500; K1725020.I13500 all in pSB3K3 in DH5a cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]