Difference between revisions of "Part:BBa J61035"
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Selectable marker inserted between EcoRI and XbaI restriction sites. The sequence inluded describes plasmid pSB1A2-b0034 (ribosome binding site part) with this cassette inserted between EcoRI and XbaI. This part facilitates the transfer of small parts during biobrick assembly. Because it is fairly large in size, EcoRI/SpeI digests of the plasmid can be readily gel purified. Because it contains a selectable marker, cloning products containing the cassette can be selected on gentamicin plates. | Selectable marker inserted between EcoRI and XbaI restriction sites. The sequence inluded describes plasmid pSB1A2-b0034 (ribosome binding site part) with this cassette inserted between EcoRI and XbaI. This part facilitates the transfer of small parts during biobrick assembly. Because it is fairly large in size, EcoRI/SpeI digests of the plasmid can be readily gel purified. Because it contains a selectable marker, cloning products containing the cassette can be selected on gentamicin plates. | ||
Revision as of 04:34, 22 November 2006
EcoRI-GenR-XbaI
Selectable marker inserted between EcoRI and XbaI restriction sites. The sequence inluded describes plasmid pSB1A2-b0034 (ribosome binding site part) with this cassette inserted between EcoRI and XbaI. This part facilitates the transfer of small parts during biobrick assembly. Because it is fairly large in size, EcoRI/SpeI digests of the plasmid can be readily gel purified. Because it contains a selectable marker, cloning products containing the cassette can be selected on gentamicin plates.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1
Illegal EcoRI site found at 2058
Illegal XbaI site found at 3533 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2058
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2514 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2058
Illegal BglII site found at 2750
Illegal BglII site found at 3487
Illegal XhoI site found at 3354 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2
Illegal EcoRI site found at 2058
Illegal XbaI site found at 3533 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2058
Illegal XbaI site found at 3533
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1097