Difference between revisions of "Part:BBa K1725000"
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<partinfo>BBa_K1725000 short</partinfo> | <partinfo>BBa_K1725000 short</partinfo> | ||
The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020. | The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020. | ||
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| + | https://static.igem.org/mediawiki/2015/thumb/a/ad/2015-08-05_10.50.30_cropped.jpg/800px-2015-08-05_10.50.30_cropped.jpg | ||
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| + | From left to right: I13500(B0034.E0040); R0040.I13500; K1725000.I13500; K1725020.I13500 all in pSB3K3 in DH5a cells. | ||
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Revision as of 17:50, 6 August 2015
PhlF repressible promoter
The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.
From left to right: I13500(B0034.E0040); R0040.I13500; K1725000.I13500; K1725020.I13500 all in pSB3K3 in DH5a cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]