Difference between revisions of "Part:BBa K1648001"
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Revision as of 16:36, 14 July 2015
Recombination Template for mamXY, mamGC and mms Operons
This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1333
Illegal BamHI site found at 432 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 603
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 875
Illegal NgoMIV site found at 1283
Illegal AgeI site found at 486 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 470
Illegal BsaI site found at 569
Illegal BsaI site found at 850