Difference between revisions of "Part:BBa K1638007"
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This part codes for a GFP reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression.
 
This part codes for a GFP reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression.
 
This part also contains two transcriptional terminators.  
 
This part also contains two transcriptional terminators.  
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====Fluorescence wavelengths====
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*'''Excitation max''' - 501nm
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*'''Emission max''' - 511nm
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For further description of the GFP: <partinfo>BBa_K1638007</partinfo>
  
 
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Revision as of 13:33, 9 July 2015

GFP reporter with flexible linker

This part codes for a GFP reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression. This part also contains two transcriptional terminators.

Fluorescence wavelengths

  • Excitation max - 501nm
  • Emission max - 511nm

For further description of the GFP: BBa_K1638007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674