Difference between revisions of "Part:BBa K1638000"
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<partinfo>BBa_K1638000 short</partinfo> | <partinfo>BBa_K1638000 short</partinfo> | ||
| − | A regulator | + | A regulator sensitive to the Lac Repressor (encoded by the regulator gene LacI) and the catabolite activator protein (CAP). |
| − | The regulator contains two binding sites. The first binds the CAP protein, which is generally | + | The regulator contains two binding sites. The first binds the CAP protein, which is generally present in E. coli and is asocciated with cell Health and availbaility of glucose. |
The second binds Lacl protein. | The second binds Lacl protein. | ||
Revision as of 12:59, 8 July 2015
Lac operator
A regulator sensitive to the Lac Repressor (encoded by the regulator gene LacI) and the catabolite activator protein (CAP).
The regulator contains two binding sites. The first binds the CAP protein, which is generally present in E. coli and is asocciated with cell Health and availbaility of glucose. The second binds Lacl protein.
In the absence of Lacl protein and CAP protein, this part promotes transcription. In the presence of LacI protein and CAP protein, this part inhibits transcription. LacI can be inhibited by IPTG. LacI is coded by BBa_C0010.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]