Difference between revisions of "Part:BBa J119386:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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It was unknown whether the Tet+Red fusion protein would work as well as Tet+GFP did.  
+
It was unknown whether the Tet+Red fusion protein would work as well as Tet+GFP did.
−
 
+
  
 +
There were two point mutations found within the TetA coding region. These mutations were a change from a G to an A at position 2127, and a change from a C to a T at position 1755. These point mutations occur in the third positions of their respective codons, and do not change the resultant amino acid being translated.
  
 
===Source===
 
===Source===

Latest revision as of 20:48, 22 June 2015


tCloneTetRed; Fusion Protein Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1266
    Illegal NgoMIV site found at 1634
    Illegal NgoMIV site found at 1794
    Illegal AgeI site found at 2741
    Illegal AgeI site found at 2853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
    Illegal BsaI.rc site found at 42


Design Notes

It was unknown whether the Tet+Red fusion protein would work as well as Tet+GFP did.

There were two point mutations found within the TetA coding region. These mutations were a change from a G to an A at position 2127, and a change from a C to a T at position 1755. These point mutations occur in the third positions of their respective codons, and do not change the resultant amino acid being translated.

Source

The part comes from J119361 tCloneRed and J119140 TetA. The linker sequence was developed from K598018 tetA+GFP fused protein by Qingyang XIAO.

References