Difference between revisions of "Part:pSB1A7"

Revision as of 17:01, 16 November 2006

Transcriptionally insulated high copy BioBrick plasmid

pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.

pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
Illegal XbaI site found at 2426
Illegal SpeI site found at 1
Illegal PstI site found at 15
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
Illegal SpeI site found at 1
Illegal PstI site found at 15
Illegal NotI site found at 8
Illegal NotI site found at 2417
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2411
Plasmid lacks a suffix.
Illegal XbaI site found at 2426
Illegal SpeI site found at 1
Illegal PstI site found at 15
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1312

@@ Line 3: / Line 3: @@
 pSB1A7 was constructed by adding double terminators ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in [https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=pSB1A3 pSB1A3]. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.
+pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.
 <!-- Add more about the biology of this part here