Difference between revisions of "Part:BBa J100210"

Latest revision as of 20:34, 2 June 2015

tCloneRed with RS4 Transcriptional Riboswitch

Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Red (J119361) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine. Based on the previous research of Wachsmuth, et al., we do not expect this to be a very effective riboswitch (small dynamic range). We will be using it as a negative control.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 761
Illegal AgeI site found at 873
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_J100210 short</partinfo>
-Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Red (J119361) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine.
+Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Red (J119361) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine. Based on the previous research of Wachsmuth, et al., we do not expect this to be a very effective riboswitch (small dynamic range). We will be using it as a negative control.
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