Difference between revisions of "Part:BBa M45697"
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coding sequence for insulin degrading enzyme | coding sequence for insulin degrading enzyme | ||
+ | Cutting sites for EcoRI and SpeI were detected and removed by NEB cutter and DNA 2.0 | ||
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Revision as of 19:51, 17 April 2015
Human insulin-degrading enzyme (IDE)
coding sequence for insulin degrading enzyme Cutting sites for EcoRI and SpeI were detected and removed by NEB cutter and DNA 2.0
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 72
Illegal NheI site found at 1896 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 354
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2487