Difference between revisions of "Part:BBa M45698"

Latest revision as of 19:41, 17 April 2015

pCstA+RBS+amilGFP used to indication of low glucose concentration

This BioBrick comsists of three parts: the promoter PcstA (BBa_K118011), the RBS (BBa_B0034) and the chromoprotein amilGFP. The increase of activity by adenylate cyclase under low glucose concentrations, results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression. This promoter has been ligated to an RBS as an intermediate step of ligations. Furthermore, The chromoprotein is from the coral Acropora millepora. It naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_M45698 short</partinfo>
-This BioBrick comprises a ligation of the registry parts for the promoter PcstA (BBa_K118011) and the RBS (BBa_B0034).
+This BioBrick comsists of three parts: the promoter PcstA (BBa_K118011), the RBS (BBa_B0034) and the chromoprotein amilGFP. The increase of activity by adenylate cyclase under low glucose concentrations, results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression. This promoter has been ligated to an RBS as an intermediate step of ligations. Furthermore, The chromoprotein is from the coral Acropora millepora.  It naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.
-The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression (more information on this part can be found on its registry page here).
-This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit.
-This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.