Difference between revisions of "Part:BBa J119376:Experience"

(Applications of BBa_J119376)
(Applications of BBa_J119376)
 
Line 6: Line 6:
 
Below are images and measurements of promoters strengths for Psimp1 and four variants of the pClone -35 Consensus cloned into it. E. coli strain JM109 was transformed with constructs that included the Psimp1 promoter variants cloned into pClone Red (BBa_J119137).  The images were taken of the resulting colonies on a UV box. The numbers below each image are the relative strengths of the Psimp1 variants measured with RFP fluorometry.
 
Below are images and measurements of promoters strengths for Psimp1 and four variants of the pClone -35 Consensus cloned into it. E. coli strain JM109 was transformed with constructs that included the Psimp1 promoter variants cloned into pClone Red (BBa_J119137).  The images were taken of the resulting colonies on a UV box. The numbers below each image are the relative strengths of the Psimp1 variants measured with RFP fluorometry.
 
<center>
 
<center>
[[File:Ptac_-35_Consensus.png|500px|]]
+
[[File:Four_variants_of_Psimp1.jpg|500px|]]
 
</center>
 
</center>
  

Latest revision as of 13:29, 27 March 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J119376

Below are images and measurements of promoters strengths for Psimp1 and four variants of the pClone -35 Consensus cloned into it. E. coli strain JM109 was transformed with constructs that included the Psimp1 promoter variants cloned into pClone Red (BBa_J119137). The images were taken of the resulting colonies on a UV box. The numbers below each image are the relative strengths of the Psimp1 variants measured with RFP fluorometry.

Four variants of Psimp1.jpg

User Reviews

UNIQb587c72a6f56f2d8-partinfo-00000000-QINU UNIQb587c72a6f56f2d8-partinfo-00000001-QINU