Difference between revisions of "Part:BBa J119375"
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Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry. | Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry. | ||
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Revision as of 02:34, 27 February 2015
81 Mutated Ptac Promoters with Varying Strengths
Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]