Difference between revisions of "Part:BBa J100198"
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<partinfo>BBa_J100198 short</partinfo> | <partinfo>BBa_J100198 short</partinfo> | ||
| − | Cloned in the fitness gene tester construct ([[Part:BBa_J100199]]), part J100198 contains the fitness gene thymidylate synthase [[Part:BBa_J100132]]. J100198 contains the a theophylline riboswitch downstream of T5, a constitutive promoter [[Part:BBa_J100132]]. The construct is built to be used in thymine auxotrophs that necessitate added thymine in the growth media for cell division. Upon successful binding of theophylline on the riboswitch (we used 0.3mM), thymidylate synthase is expressed for prototophic growth and cellular division. | + | Cloned in the fitness gene tester construct ([[Part:BBa_J100199]]), part J100198 contains the fitness gene thymidylate synthase [[Part:BBa_J100132]]. J100198 contains the a theophylline riboswitch downstream of T5, a constitutive promoter [[Part:BBa_J100132]]. The construct is built to be used in thymine auxotrophs that necessitate added thymine in the growth media for cell division. Upon successful binding of theophylline on the riboswitch (we used 0.3mM), thymidylate synthase is expressed for prototophic growth and cellular division. J100198 was cloned into pSB1A8 [[Part:BBa_J119043]]. |
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Revision as of 14:52, 20 December 2014
J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes
Cloned in the fitness gene tester construct (Part:BBa_J100199), part J100198 contains the fitness gene thymidylate synthase Part:BBa_J100132. J100198 contains the a theophylline riboswitch downstream of T5, a constitutive promoter Part:BBa_J100132. The construct is built to be used in thymine auxotrophs that necessitate added thymine in the growth media for cell division. Upon successful binding of theophylline on the riboswitch (we used 0.3mM), thymidylate synthase is expressed for prototophic growth and cellular division. J100198 was cloned into pSB1A8 Part:BBa_J119043.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 995 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 995 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 995 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 995
Illegal AgeI site found at 107
Illegal AgeI site found at 770 - 1000COMPATIBLE WITH RFC[1000]