Difference between revisions of "Part:BBa J100198:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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This part works as designed. thyA- cells that contain J100198 do not grow unless theophylline is added to the media. Growth rates are comparable with 0.3, 0.6 and 1.25 mM theophylline added to LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation).
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how you used this part and how it worked out.
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[[File:cellsNtubes.png]]
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Photograph of ''thyA-'' cells that contain J100198 in pSB1A8 grown in  LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). From left to right: 0, 0.3, 0.6 and 1.25 mM theophylline.
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[[File:Cells_graphed..png]]
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OD<sub>590</sub> of ''thyA-'' cells that contain J100198 in pSB1A8 grown in  LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation) with added theophylline as indicated. The cells were grown overnight and measured then ext morning.  
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===Applications of BBa_J100198===
 
===Applications of BBa_J100198===

Revision as of 13:34, 20 December 2014

This part works as designed. thyA- cells that contain J100198 do not grow unless theophylline is added to the media. Growth rates are comparable with 0.3, 0.6 and 1.25 mM theophylline added to LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation).

CellsNtubes.png Photograph of thyA- cells that contain J100198 in pSB1A8 grown in LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). From left to right: 0, 0.3, 0.6 and 1.25 mM theophylline.


Cells graphed..png OD590 of thyA- cells that contain J100198 in pSB1A8 grown in LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation) with added theophylline as indicated. The cells were grown overnight and measured then ext morning. </center>


Applications of BBa_J100198

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