Difference between revisions of "Part:BBa M36900:Experience"
| Line 3: | Line 3: | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | Barcode #: 133018572 | + | Barcode # (glycerol stock): 133018572 |
Plasmid Name: NeginandAbbey | Plasmid Name: NeginandAbbey | ||
| Line 14: | Line 14: | ||
Sensor | Sensor | ||
| + | |||
| + | In experimentation of this part's performance, our goals were to establish whether or not the device at hand would perform as a functional salt sensor in eukaryotic HeLa cells, by quantifying fluorescence level outputs actuated by a coupled actuator for various levels of salt stress input, both in terms of stress duration and NaCl concentration in the solution. At all experimental conditions (40,80,120 micromolar NaCl solution x 35, 70 min exposure time) our transformed HeLa cells exhibited significantly larger levels of fluorescence after salt stress exposure, as compared to the mock-transfected cells exposed to identical environment. However, | ||
| + | |||
| + | Please see the attached .pdf for details on device performance as a dose response curve over two ranges of mercury ion concentration. In the graphs, red bars represent growth of cells with the BBa_M36567 construct and blue bars represent growth of normal E.coli K12 cells with no mercury reduction plasmid | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:52, 10 December 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Barcode # (glycerol stock): 133018572
Plasmid Name: NeginandAbbey
Antibiotic Resistance: Ampicillin
DNA 2.0 Gene #: 193570
Organism Expressed in HeLa
Sensor
In experimentation of this part's performance, our goals were to establish whether or not the device at hand would perform as a functional salt sensor in eukaryotic HeLa cells, by quantifying fluorescence level outputs actuated by a coupled actuator for various levels of salt stress input, both in terms of stress duration and NaCl concentration in the solution. At all experimental conditions (40,80,120 micromolar NaCl solution x 35, 70 min exposure time) our transformed HeLa cells exhibited significantly larger levels of fluorescence after salt stress exposure, as compared to the mock-transfected cells exposed to identical environment. However,
Please see the attached .pdf for details on device performance as a dose response curve over two ranges of mercury ion concentration. In the graphs, red bars represent growth of cells with the BBa_M36567 construct and blue bars represent growth of normal E.coli K12 cells with no mercury reduction plasmid
User Reviews
UNIQ318a5c5a0b8b52d9-partinfo-00000000-QINU UNIQ318a5c5a0b8b52d9-partinfo-00000001-QINU