Difference between revisions of "Part:BBa M36019:Experience"
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| − | From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. | + | From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups. |
[[File: assay results.png]] | [[File: assay results.png]] | ||
- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727 | - Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727 | ||
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- Plasmid name: somcrl1 | - Plasmid name: somcrl1 | ||
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- Antibiotic resistance: amp | - Antibiotic resistance: amp | ||
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- DNA 2.0 gene # 193463 | - DNA 2.0 gene # 193463 | ||
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- Organism expressed in: E. Coli | - Organism expressed in: E. Coli | ||
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- Sensor for lactic acid | - Sensor for lactic acid | ||
Revision as of 23:36, 5 December 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36019
Stanford Location By: Melissa, Sydney, Rachel (BIOE 44 Fall 2014) Experience:
From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups.
- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727
- Plasmid name: somcrl1
- Antibiotic resistance: amp
- DNA 2.0 gene # 193463
- Organism expressed in: E. Coli
- Sensor for lactic acid
User Reviews
UNIQ7579dd7a0eeec703-partinfo-00000000-QINU UNIQ7579dd7a0eeec703-partinfo-00000001-QINU