Difference between revisions of "Part:BBa M36921"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The promoter and ZntR regulatory gene of the znt operon are naturally existing in E. coli and were first sequenced and amplified from E. coli DH5a genomic DNA. | + | We transfected our plasmid into an ''E. coli'' cell line and wanted to employ it as a primary screening technique for optimum concentrations of Zn2+ in soil. The promoter and ZntR regulatory gene of the znt operon are naturally existing in E. coli and were first sequenced and amplified from E. coli DH5a genomic DNA. |
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Revision as of 06:29, 5 December 2014
Zinc Sensor
This composite part contains a constitutive promoter, ribosome-binding-site, a gene for ZntR, an E. Coli terminator, and a promoter that is ZntR regulated. In the absence of Zinc ions, the ZntR protein binds to the ZntR promoter binding site. In the presence of zinc ions, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of our actuator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 194
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 194 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 190
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 194
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 194
- 1000COMPATIBLE WITH RFC[1000]