Difference between revisions of "Part:BBa K1470005"

Revision as of 22:19, 2 November 2014

engineered PDZ domain (ePDZ)

Usage and Biology

The engineered PDZ domain is a globuar protein domain of 80 - 90 amino acids wich has an enhanced interaction with other proteins. Normally PDZ domains bind to a short region at the C-terminus of other proteins but there are some proteins which recognize internal sequence motifs of target proteins through a single binding site on the domains [1][2]. There are more over 1000 known proteins containing this domain in eucaryotes and bacteria but just a few examples are shown in archea [3].
ePDZ is a crucial part of the blue light-inducible expression system. It is able to bind to the J-alpha-helix of the LOV2 domain. The principle of the light-induced expression is explained <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1150005">here</a>.

References

[1] Cowburn D (December 1997). „Peptide recognition by PTB and PDZ domains“. Curr. Opin. Struct. Biol. 7 (6): 835–838
[2] Giallourakis C, Cao Z, Green T, Wachtel H, Xie X, Lopez-Illasaca M, Daly M, Rioux J, Xavier R. A molecular-properties-based approach to understanding PDZ domain proteins and PDZ ligands. Genome Res. 2006;16:1056–1072.
[3] Doyle DA, Lee A, Lewis J, Kim E, Sheng M, MacKinnon R (June 1996). „Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ“. Cell 85 (7): 1067–1076

Sequence and Features