Difference between revisions of "Part:BBa K1471012:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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We tried the system in a prokaryote chassis, e. coli.
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The entire system was ensambled with 3A assembly and including two genes from the mer operon and also a GFP reporter which was used to measure the expression of the system.
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===Source===
 
===Source===

Latest revision as of 21:27, 2 November 2014


RBS - MerE - RBS - MerB - RBS - GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1511


Design Notes

The entire system was ensambled with 3A assembly and including two genes from the mer operon and also a GFP reporter which was used to measure the expression of the system.

Source

Mer genes comes from bacterial genomic sequences

References