Difference between revisions of "Part:BBa K1499250"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1499250 short</partinfo> | <partinfo>BBa_K1499250 short</partinfo> | ||
Line 5: | Line 4: | ||
todo | todo | ||
− | <!-- | + | <!--This part is GFP with two inserted TAG stop codons in place of leucine residues. This part transformed into Amberless cells along with supP tRNA will express GFP. In wild type cells, no GFP will be expressed as the TAG will be recognized as stop codons rather than leucine. This part is used as a proof of concept for Codon Security which mitigates the effects of horizontal gene transfer by making these genes unreadable by natural organisms. |
+ | <div class="small-7 small-centered columns"><br><center><img src="https://static.igem.org/mediawiki/2014/a/a4/SBSiGEM_HellCell1.png"><br> | ||
+ | <h6><b>Figure 1.</b> Our approach to the Amberless Hell Cell. By recoding the UAG stop codon to translate into an amino acid, only cells that have a tRNA with the anticodon AUC will produce the complete protein. In our experiment, we used a tRNA that charges with leucine to translate the UAG codon.</h6> | ||
+ | </div><br> | ||
+ | <div class="row"> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:23, 2 November 2014
GFP (E0040) with two amber stop codons
todo
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705