Difference between revisions of "Part:BBa K1398007"
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https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg
 
https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg
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The synthetic promoter has a higher background fluorescence activity than the control cells. The activity of the synthetic promoter rises to around the level of the native NemR promoter with exposure to trinitrotoluene.
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The full article can be found here:
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http://2014.igem.org/Team:Exeter/Detection
  
 
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Revision as of 19:02, 2 November 2014

NemR Promoter Reporter

A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008). The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.

Fluorescence response per OD is displayed below:

Exeter2014_007_InducableTNTpromoter.jpg

The synthetic promoter has a higher background fluorescence activity than the control cells. The activity of the synthetic promoter rises to around the level of the native NemR promoter with exposure to trinitrotoluene.

The full article can be found here:

http://2014.igem.org/Team:Exeter/Detection

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]