Difference between revisions of "Part:BBa K1477030:Design"

 
(→‎Design Notes)
 
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===Design Notes===
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===Design Notes==
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N/A
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This part was built to short circuit bacteriophage infection in a host cell; Infection by phage was designed to result in immediate lysis with no procession of the infectious cycle.
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<p>A typical phage replication cycle is represented here:
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[[File:Phage_life_cycle.png‎]]
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</p>
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<p>BBa_K1477030 was designed to short circuit coliphage T7 infection
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[[File:Phage_Immediate_Lysis.png‎]]
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</p>
  
 
===Source===
 
===Source===

Latest revision as of 16:53, 2 November 2014


T7 strong promoter + endolysin cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 345
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


=Design Notes

This part was built to short circuit bacteriophage infection in a host cell; Infection by phage was designed to result in immediate lysis with no procession of the infectious cycle.

A typical phage replication cycle is represented here: Phage life cycle.png

BBa_K1477030 was designed to short circuit coliphage T7 infection Phage Immediate Lysis.png

Source

T7 virus and Endolysin

References