Difference between revisions of "Part:BBa K1321337"

Revision as of 05:33, 2 November 2014

sfGFP in Freiburg format (RFC 25)

sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins. We used the part I746909 as our template for PCR with primers that use overhanging ends to insert the Freiburg sites.

Usage and Biology

Superfolder Green Fluorescent Protein (sfGFP) is a mutant of mut3b GFP (E0040) with improved in vivo properties. It was first entered into the registry by the Cambridge iGEM team in 2008; please see I746916 part description and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP for detailed information about the properties of sfGFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 10

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 <partinfo>BBa_K1321337 short</partinfo>
-sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
+sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins. We used the part I746909 as our template for PCR with primers that use overhanging ends to insert the Freiburg sites.
 ===Usage and Biology===
 Superfolder Green Fluorescent Protein (sfGFP) is a mutant of mut3b GFP (E0040) with improved in vivo properties. It was first entered into the registry by the Cambridge iGEM team in 2008; please see I746916 part description and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP for detailed information about the properties of sfGFP.
+[[File:IC-2014_glow1.jpg |400px|centre|]]
+[[File:IC-2014_glow2.jpg |400px|centre|]]
 ===Sequence and Features===