Difference between revisions of "Part:BBa K1321103"
Line 7: Line 7:
  
 
Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
 
Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
−
 
−
For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen [https://parts.igem.org/Part:BBa_K1321367 here]  - K1321371.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:38, 2 November 2014

Phytochelatin (PC) EC20 + linker-CBDcipA-linker with T7 promoter

A T7-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 335
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]