Difference between revisions of "Part:BBa K1314000:Experience"
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Latest revision as of 04:11, 2 November 2014
Contents
EcoRI PstI double digest check the gel photo here shows the expected band size of 250 bp of insert: nifH promoter
Applications of BBa_K1314000
Expressing nifH promoter(Bba_K1314000) with BBa_E1010 in E. coli DH5a
Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a.
Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ
User Reviews
It is a promoter designed for Azotobacter vinelandii. It is not functional in E. coli. UNIQ25d37f9e1273a0a8-partinfo-00000001-QINU UNIQ25d37f9e1273a0a8-partinfo-00000002-QINU