Difference between revisions of "Part:BBa K1314000:Experience"

Revision as of 03:52, 2 November 2014

EcoRI PstI double digest check the gel photo here shows the expected band size of 250 bp of insert: nifH promoter

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-__NOTOC__
+<html><img src="https://static.igem.org/mediawiki/parts/7/70/K1314000gelphotos.JPG"></html>
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
+EcoRI PstI double digest check
+the gel photo here shows the expected band size of 250 bp of insert: nifH promoter
 ===Applications of BBa_K1314000===
+====Expressing nifH promoter(Bba_K1314000) with BBa_E1010 in E. coli DH5a====
+Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a.
+[[File:NifH E1010.jpg|200px]]
+===Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ===
+[[File:Picture2.png|800px]]
+<!-- -->
 ===User Reviews===
+It is a biobricks designed for Azotobacter vinelandii. It is not functional in E. coli.
 <!-- DON'T DELETE --><partinfo>BBa_K1314000 StartReviews</partinfo>
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