Difference between revisions of "Part:BBa K1363601"

Latest revision as of 03:35, 2 November 2014

the gate of yes of RNA logic gates

The ribozyme which loses its activity for secondary structure is set in front of a GFP, promoted by a continuous promoter, while a short RNA, the key of yes, is promoted by a Plac. When lac is added, the short RNA is expressed and activate the ribozyme. The cleavage is induced and the expression of protein declines.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 116
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 116
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 116
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1363601 short</partinfo>
-the gate RNA will be activited when combined with the short RNA key-of-yes
+<html>
+<style>
+p { font-size: large; }
+</style>
+      <p>The ribozyme which loses its activity for secondary structure is set in front of a GFP, promoted by a continuous promoter, while a short RNA, the key of yes, is promoted by a Plac. When lac is added, the short RNA is expressed and activate the ribozyme. The cleavage is induced and the expression of protein declines.</p>
+      <figure style="text-align:center"><img src="https://static.igem.org/mediawiki/2014/a/a7/Ustc-2014-rna-YESh.png" width="500" class="th" \><figcaption>
+Fig.5 The principle of YES gate.
+      </figcaption></figure>
+      <figure style="text-align:center"><img src="https://static.igem.org/mediawiki/2014/4/47/Ustc-2014-rna-YES0.png" width="600" class="th" \><figcaption>
+Fig.6 The schematic of YES gate.
+      </figcaption></figure>
+</html>
 <!-- Add more about the biology of this part here