Difference between revisions of "Part:BBa K1363401"

 
 
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<partinfo>BBa_K1363401 short</partinfo>
 
<partinfo>BBa_K1363401 short</partinfo>
  
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This part is the downstream passway of the red color system.When the upstream passway senses the red light,it will open the express of RFP and when there is no light,the promoter of RFP will be inhibited.
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<h3>Introduction</h3>
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<p>In the red light-inducible system, The SK Cph8 is produced in a kinase-active Pr state . 
In dark, Chp8 phosphorylates the response regulator OmpR, activating the transcription from the ompC promoter thus producing more lambda CI. The increase of lambda CI repress its transcription of downstream promoter for RFP. Red light switches Cph8 Pr to a far red–absorbing state (Pfr), which dephosphorylates phospho-OmpR, deactivating the transcription of OmpR. So the inverter has no effect and RFPs are continuously transcribed.</p>
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<img src="https://static.igem.org/mediawiki/parts/a/ab/USTC-3401.png" width="400" />
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<img src="https://static.igem.org/mediawiki/parts/4/41/USTC-3401B.png" width="400" />
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:32, 2 November 2014

the downstream passway of the red color system

Introduction

In the red light-inducible system, The SK Cph8 is produced in a kinase-active Pr state . 
In dark, Chp8 phosphorylates the response regulator OmpR, activating the transcription from the ompC promoter thus producing more lambda CI. The increase of lambda CI repress its transcription of downstream promoter for RFP. Red light switches Cph8 Pr to a far red–absorbing state (Pfr), which dephosphorylates phospho-OmpR, deactivating the transcription of OmpR. So the inverter has no effect and RFPs are continuously transcribed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1568
    Illegal AgeI site found at 1680
  • 1000
    COMPATIBLE WITH RFC[1000]