Difference between revisions of "Part:BBa K1363200"

 
 
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<partinfo>BBa_K1363200 short</partinfo>
 
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We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac.  
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<h3>Results</h3>
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<p>We didn’t success in constructing this part. However, we directly complete the final circuit, which consists of lactose operator and signal peptide. Then we test it and it works well:</p>
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<figure><img src="https://static.igem.org/mediawiki/parts/2/2c/USTC-3200.png" width="400"/><figcaption>We used gradient plate method in our experiment. As we can see in the picture, the concentration of IPTG on the left of the medium is 0.1% while the concentration on the right is zero. We can see colony only on the right of the medium, the kill switch is effective which illustrates that IPTG can induce the expression of signal peptide and LALF so as to inhibit the growth of the E.coil. Also, a decrease of OD is observed too, after we added IPTG to the liquid culture medium, which means our kill switch did work well.</figcaption></figure>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:26, 2 November 2014

Anti-LPS factor(LALF) regulated by lacI

Results

We didn’t success in constructing this part. However, we directly complete the final circuit, which consists of lactose operator and signal peptide. Then we test it and it works well:

We used gradient plate method in our experiment. As we can see in the picture, the concentration of IPTG on the left of the medium is 0.1% while the concentration on the right is zero. We can see colony only on the right of the medium, the kill switch is effective which illustrates that IPTG can induce the expression of signal peptide and LALF so as to inhibit the growth of the E.coil. Also, a decrease of OD is observed too, after we added IPTG to the liquid culture medium, which means our kill switch did work well.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]