Difference between revisions of "Part:BBa K1363001"
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</p> | </p> | ||
<h3>Get the part</h3> | <h3>Get the part</h3> | ||
| − | <figure><img src="https://static.igem.org/mediawiki/parts/7/7e/USTC-parts-3.png" width="400"/><figcaption>Fig | + | <figure><img src="https://static.igem.org/mediawiki/parts/7/7e/USTC-parts-3.png" width="400"/><figcaption>Fig 1</figcaption></figure> |
<p>We get this part by PCR its gene from the genome of C.Crescentus. The standard parts construction have been accomplished, which helps motion regulation and holdfast biosynthesis of bacteria.</p> | <p>We get this part by PCR its gene from the genome of C.Crescentus. The standard parts construction have been accomplished, which helps motion regulation and holdfast biosynthesis of bacteria.</p> | ||
<h3>Reference</h3> | <h3>Reference</h3> | ||
Latest revision as of 02:27, 2 November 2014
DgrB the receptor of c-di-GMP
Introduction
The concentration of c-di-GMP is varying, and for our experiment we will promote its expression to inhibit rotation of flagella. It will bind to DgrA and DgrB. The resulting complex will have two routes to go: one is the [c-di-GMP& DgrA] complex which will inhibit FliL, a protein bounds to the membrane playing a key role in rotation of flagella. The other route is that the DgrB will directly affect the rotation of the flagella.
Get the part
We get this part by PCR its gene from the genome of C.Crescentus. The standard parts construction have been accomplished, which helps motion regulation and holdfast biosynthesis of bacteria.
Reference
DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 162
Illegal NgoMIV site found at 288 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 35