Difference between revisions of "Part:BBa K1363002"

 
 
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<partinfo>BBa_K1363002 short</partinfo>
 
<partinfo>BBa_K1363002 short</partinfo>
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<h3>Introduction</h3>
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<p>HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]</p>
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<figure><img src="https://static.igem.org/mediawiki/parts/6/61/USTC-7.png" width="400" /><figcaption>Fig 1</figcaption></figure>
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<p>These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.</p>
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<p>These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.</p>
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<figure><img src="https://static.igem.org/mediawiki/parts/1/19/USTC-6.png" width="500" /><figcaption>Fig 2</figcaption></figure>
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<h3>Get this part</h3>
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<p>To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it, sequenced it and the result is exactly right. </p>
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<img src="https://static.igem.org/mediawiki/parts/3/3f/USTC-8.png" width="400" />
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<figcaption> PCR result: HfiA together with two another motion control proteins-DgrA and DgrB </figcaption></figure>
  
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HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway.It can inhibit one of the Hfs protein family-HfsJ.So it can block up the synthesis of polysaccharide.Without the polysaccharide,Caulobacter crescentus can hardly stick to the surface.We use this principle to regulate the Caulobacter crescentus's movement.
 
  
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 02:21, 2 November 2014

HfiA the Inhibitor of synthesis of polysaccharose

Introduction

HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]

Fig 1

These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.

These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.

Fig 2

Get this part

To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it, sequenced it and the result is exactly right.

PCR result: HfiA together with two another motion control proteins-DgrA and DgrB
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]