Difference between revisions of "Part:BBa K1363001"

 
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The DgrB is a protein which firstly be discovered in the G- bacteria Caulobacter crescentus.It is a receptor of c-di-GMP and its function is to inhibit the movement of flagellum.We use it to inhibit the movement of the flagellum of Caulobacter crescentus when it recepts the illumination to let the bacteria adhere in time.
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<h3>Introduction</h3>
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<p>The concentration of c-di-GMP is varying, and for our experiment we will promote its expression to inhibit rotation of flagella. It will bind to DgrA and DgrB. The resulting complex will have two routes to go: one is the [c-di-GMP&amp; DgrA] complex which will inhibit FliL, a protein bounds to the membrane playing a key role in rotation of flagella. The other route is that the DgrB will directly affect the rotation of the flagella.
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<figure><img src="https://static.igem.org/mediawiki/parts/8/8c/USTC-parts-2.png" width="400"/><figcaption>Fig 1: A dimer of ci-d-GMP binds to the surface of a homolog of DgrA </figcaption></figure>
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<h3>Get the part</h3>
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<figure><img src="https://static.igem.org/mediawiki/parts/7/7e/USTC-parts-3.png" width="400"/><figcaption>Fig 2</figcaption></figure>
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<p>We get this part by PCR its gene from the genome of C.Crescentus. The standard parts construction have been accomplished, which helps motion regulation and holdfast biosynthesis of bacteria.</p>
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<h3>Reference</h3>
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<p><em>DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus</em></p>
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Revision as of 02:19, 2 November 2014

DgrB the receptor of c-di-GMP

Introduction

The concentration of c-di-GMP is varying, and for our experiment we will promote its expression to inhibit rotation of flagella. It will bind to DgrA and DgrB. The resulting complex will have two routes to go: one is the [c-di-GMP& DgrA] complex which will inhibit FliL, a protein bounds to the membrane playing a key role in rotation of flagella. The other route is that the DgrB will directly affect the rotation of the flagella.

Fig 1: A dimer of ci-d-GMP binds to the surface of a homolog of DgrA

Get the part

Fig 2

We get this part by PCR its gene from the genome of C.Crescentus. The standard parts construction have been accomplished, which helps motion regulation and holdfast biosynthesis of bacteria.

Reference

DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 162
    Illegal NgoMIV site found at 288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 35