Difference between revisions of "Part:BBa K1332010"
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<partinfo>BBa_K1332010 short</partinfo>
 
<partinfo>BBa_K1332010 short</partinfo>
  
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This part consists of the 5’ side of the intron in td gene of T4 phage and a double terminator. The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side) can be circularized. The 5´side of the intron in td gene of T4 phage contains a stop codon, so a circular mRNA made by this part <b>doesn’t cause continuous translation.</b>
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This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage([https://parts.igem.org/Part:BBa_K1332004 BBa_K1332004]) and a double terminator([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008]) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part <b>doesn’t cause continuous translation.</b>
  
 
<h3>How to use</h3>
 
<h3>How to use</h3>
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Insert the protein coding sequence between this device and mRNA circularization device (5’ side).
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Insert the protein coding sequence between this device and mRNA circularization device (5’ side)([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008]).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:09, 2 November 2014

mRNA circularization device (3 side)

This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage(BBa_K1332004) and a double terminator(BBa_B0015). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)(BBa_K1332008) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.

How to use

Insert the protein coding sequence between this device and mRNA circularization device (5’ side)(BBa_K1332008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]