Difference between revisions of "Part:BBa K1555000"
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This part is our testing part.The ''copa'' is one of the candidates of our copper sensor.For comparing with other two sequences,''marO''（BBa_K1555001） and ''cueo''（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. | This part is our testing part.The ''copa'' is one of the candidates of our copper sensor.For comparing with other two sequences,''marO''（BBa_K1555001） and ''cueo''（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 00:57, 2 November 2014
The copa promoter wth a gfp reporter
This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marO（BBa_K1555001） and cueo（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914