Difference between revisions of "Part:BBa K1555000"

Revision as of 00:17, 2 November 2014

File:Https://static.igem.org/mediawiki/parts/1/19/2h 0mg.L copper induce .tif The copa promoter wth a gfp reporter

This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marO（BBa_K1555001） and cueo（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. File:2h 0mg.Lcopper induce.tif File:Part:BBa K1555000 Sequence and Features

Assembly Compatibility:

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COMPATIBLE WITH RFC[25]
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Illegal BsaI.rc site found at 914

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 This part is our testing part.The ''copa'' is one of the candidates of our copper sensor.For comparing with other two sequences,''marO''（BBa_K1555001） and ''cueo''（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity.
 [[File:2h 0mg.Lcopper induce.tif]][[File:Part:BBa K1555000]]
+[[File:Kepler186f artistconcept 1.jpg]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===