Difference between revisions of "Part:BBa K1555000"

Revision as of 00:11, 2 November 2014

The copa promoter wth a gfp reporter

This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marO（BBa_K1555001） and cueo（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. File:2h 0mg.Lcopper induce.tif File:Part:BBa K1555000 Sequence and Features

Assembly Compatibility:

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COMPATIBLE WITH RFC[10]
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COMPATIBLE WITH RFC[12]
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COMPATIBLE WITH RFC[21]
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COMPATIBLE WITH RFC[23]
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COMPATIBLE WITH RFC[25]
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INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 914

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-[[Media:Part:BBa K1555000]]__NOTOC__
+__NOTOC__
 <partinfo>BBa_K1555000 short</partinfo>
 This part is our testing part.The ''copa'' is one of the candidates of our copper sensor.For comparing with other two sequences,''marO''（BBa_K1555001） and ''cueo''（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity.
-[[File:2h 0mg.L铜离子诱导（无诱导）激发.tif]][[File:Part:BBa K1555000]]
+[[File:2h 0mg.Lcopper induce.tif]][[File:Part:BBa K1555000]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===