Difference between revisions of "Part:BBa K1391107:Experience"
 
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This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur.
 
This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur.
  
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B-cell receptors (BCRs) are naturally occurring, transmembrane protein complexes that consist of a membrane-bound antibody (IgM) and some associated proteins (CD79A and CD79B). Given that the variable region of the antibody can be specific for any of a large number of antigens, we designed a B-cell receptor to bind beta-amyloid plaques (a biomolecular hallmark of Alzheimer's disease). Once bound, activated receptors instigate intracellular signalling, which can then be manipulated to diagnose the disease.
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Spleen Tyrosine Kinase (Syk) is a tyrosine kinase that has a high affinity for the phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) of the CD79A and CD79B proteins (components of the B-Cell Receptor Complex). Syk binds to the phosphorylated ITAM and the ITAM then phosphorylates Syk allowing Syk to initiate a downstream signalling cascade that ordinarily results in the proliferation of B-Cells during clonal selection.
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TEV protease is a protease that cleaves at a particular amino acid sequence. In our synthetic B-cell receptor system, a transcriptional activator (Gal4VP16) is fused using a TEV protease cleavage site to the intracellular tails of CD79A and/or CD79B. When the Syk-TEVp fusion is recruited to our engineered receptor upon antigen binding, the TEV protease cleaves at its cleavage site on the CD79A and/or CD79B fusions and releases a transcriptional activator.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 23:19, 1 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1391107

This is fusion protein comprising Syk and tobacco etch virus (TEV) protease, joined by a glycine-serine linker. This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur.

Spleen Tyrosine Kinase (Syk) is a tyrosine kinase that has a high affinity for the phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) of the CD79A and CD79B proteins (components of the B-Cell Receptor Complex). Syk binds to the phosphorylated ITAM and the ITAM then phosphorylates Syk allowing Syk to initiate a downstream signalling cascade that ordinarily results in the proliferation of B-Cells during clonal selection.

TEV protease is a protease that cleaves at a particular amino acid sequence. In our synthetic B-cell receptor system, a transcriptional activator (Gal4VP16) is fused using a TEV protease cleavage site to the intracellular tails of CD79A and/or CD79B. When the Syk-TEVp fusion is recruited to our engineered receptor upon antigen binding, the TEV protease cleaves at its cleavage site on the CD79A and/or CD79B fusions and releases a transcriptional activator.

User Reviews

UNIQ78f941a73e6ea18a-partinfo-00000000-QINU UNIQ78f941a73e6ea18a-partinfo-00000001-QINU